GSM6611387: dC-C-scramble-CTCF; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
CTCF
Cell type
Cell type Class
Kidney
Cell type
293
Primary Tissue
Kidney
Tissue Diagnosis
Normal
Attributes by original data submitter
Sample
source_name
HEK293T
cell line
HEK293T
cell type
Human embryonic kidney
chip antibody
CTCF (Abcam, #ab128873)
geo_loc_name
missing
collection_date
missing
Sequenced DNA Library
library_name
GSM6611387
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were fixed with 1% formaldehyde for 10 minutes. After quenching and cell lysis, chromatin was sonicated using a focused-ultrasonicator (Covaris). ChIP-seq libraries were prepared using the NEBNext® Ultra™ II DNA Library Prep Kit (NEB #E7645). 200 ng of DNA was used as starting material for input and 4-60 ng for ip samples. length using Agencourt AMPure XP beads from Beckman Coulter. Libraries were validated using the Agilent High Sensitivity DNA Kit.